DNA Degradation Study

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Sea water was collected at the depth of a typical offshore outfall
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Water samples were spiked with wastewater treatment plant effluent that had undergone different levels of chlorination and UV disinfection
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Some samples were kept under temperature control
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Degradation of pathogens in the samples was measured over time
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Analyses were conducted at regular intervals over several days
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Both rapid and traditional microbial monitoring techniques were used to analyze the samples
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Some samples were prepared at SCCWRP and analyzed offsite
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Dr. Yiping Cao of SCCWRP
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Hollow fiber filtration (HFF) apparatus used to prepare samples for rapid analysis of Enterococcus and Bacteriodes using QPCR and PMA-PCR
Please credit images to SCCWRP.